xform is designed to operate on a variety of data types, especially objects encapsulating neurons. xform depends on two specialised downstream functions xformpoints and xformimage. These are user visible any contain some useful documentation, but should only be required for expert use; in almost all circumstances, you should use only xform.

xform.character is designed to work with files on disk. Presently it is restricted to images, although other datatypes may be supported in future.

xform(x, reg, ...)

# S3 method for default
xform(x, reg, na.action = c("warn", "none", "drop",
  "error"), ...)

# S3 method for character
xform(x, reg, ...)

# S3 method for list
xform(x, reg, FallBackToAffine = TRUE,
  na.action = "error", ...)

# S3 method for shape3d
xform(x, reg, FallBackToAffine = TRUE,
  na.action = "error", ...)

# S3 method for neuron
xform(x, reg, FallBackToAffine = TRUE,
  na.action = "error", ...)

# S3 method for data.frame
xform(x, reg, subset = NULL, ...)

# S3 method for dotprops
xform(x, reg, FallBackToAffine = TRUE, ...)

# S3 method for neuronlist
xform(x, reg, subset = NULL, ...,
  OmitFailures = NA, VectoriseRegistrations = FALSE,
  TransformDFCoords = TRUE)

Arguments

x

an object to transform

reg

A registration defined by a matrix, a function, a cmtkreg object, or a character vector specifying a path to one or more registrations on disk (see Registrations section).

...

additional arguments passed to methods and eventually to xformpoints

na.action

How to handle NAs. NB drop may not work for some classes.

FallBackToAffine

Whether to use an affine transform when a cmtk warping transformation fails.

subset

For xform.neuronlist indices (character/logical/integer) that specify a subset of the members of x to be transformed.

OmitFailures

Whether to omit neurons for which FUN gives an error. The default value (NA) will result in nlapply stopping with an error message the moment there is an error. For other values, see details.

VectoriseRegistrations

When FALSE, the default, each element of reg will be applied sequentially to each element of x. When TRUE, it is assumed that there is one element of reg for each element of x.

TransformDFCoords

If the metadata data.frame attached to x includes columns that look like x,y,z coordinates, transform those as well.

Details

Methods are provided for some specialised S3 classes. Further methods can of course be constructed for user-defined S3 classes. However this will probably not be necessary if the xyzmatrix and `xyzmatrix<-` generics are suitably overloaded and the S3 object inherits from list.

Note that given the behaviour of the xyzmatrix functions, the xform.data.frame method will transform the x,y,z or X,Y,Z columns of a data.frame if the data.frame has more than 3 columns, erroring out if no such unique columns exist.

TODO get this to work for matrices with more than 3 columns by working on xyzmatrix definition.

For the xform.dotprops method, dotprops tangent vectors will be recalculated from scratch after the points have been transformed (even though the tangent vectors could in theory be transformed more or less correctly). When there are multiple transformations, xform will take care to carry out all transformations before recalculating the vectors.

With xform.neuronlist, if you want to apply a different registration to each object in the neuronlist x, then you should use VectoriseRegistrations=TRUE.

When x's attached data.frame contains columns called x,y,z or X,Y,Z then these are assumed to be coordinates and also transformed when TransformDFCoords=TRUE (the default). This provides a mechanism for transforming the soma positions of neuronlist objects containing dotprops objects (which do not otherwise store the soma position). Note that if transformation fails, a warning will be issued and the points will be replaced with NA values.

Registrations

When reg is a character vector, xform's specialised downstream functions will check to see if it defines a path to one (or more) registrations on disk. These can be of two classes

  • CMTK registrations

  • reglist objects saved in R's RDS format (see readRDS) which can contain any sequence of registrations supported by nat.

If the path does indeed point to a CMTK registration, this method will hand off to xformpoints.cmtkreg or xformimages.cmtkreg. In this case, the character vector may optionally have an attribute, 'swap', a logical vector of the same length indicating whether the transformation direction should be swapped. At the moment only CMTK registration files are supported.

If reg is a character vector of length >=1 defining a sequence of registration files on disk they should proceed from sample to reference.

Where reg is a function, it should have a signature like myfun(x,), ... where the ... must be provided in order to swallow any arguments passed from higher level functions that are not relevant to this particular transformation function.

See also

Examples

if (FALSE) { kc1=kcs20[[1]] kc1.default=xform(kc1,function(x,...) x) stopifnot(isTRUE(all.equal(kc1,kc1.default))) kc1.5=xform(kc1,function(x,...) x, k=5) stopifnot(isTRUE(all.equal(kc1.5,kc1.default))) kc1.20=xform(kc1,function(x,...) x, k=20) stopifnot(!isTRUE(all.equal(kc1,kc1.20))) # apply two registrations converting sample->IS2->JFRC2 reg_seq=c("IS2_sample.list", "JFRC2_IS2.list") xform(kc1, reg_seq) # apply two registrations, swapping the direction of the second one # i.e. sample -> IS2 -> FCWB reg_seq=structure(c("IS2_sample.list", "IS2_FCWB.list"), swap=c(FALSE, TRUE)) xform(kc1, reg_seq) } if (FALSE) { # apply reg1 to Cell07PNs[[1]], reg2 to Cell07PNs[[2]] etc regs=c(reg1, reg2, reg3) nx=xform(Cell07PNs[1:3], reg=regs, VectoriseRegistrations=TRUE) }