the ngraph class contains a (completely general) graph representation of a neuron's connectivity in an igraph object. It may additionally contain vertex name or position data. See Connectivity section.

ngraph() creates an ngraph from edge and vertex information.

as.ngraph converts an object to an ngraph

as.ngraph.dataframe construct ngraph from a data.frame containing SWC format data

as.ngraph.neuron construct ngraph from a neuron

ngraph(
el,
vertexnames,
xyz = NULL,
diam = NULL,
directed = TRUE,
weights = FALSE,
vertex.attributes = NULL,
graph.attributes = NULL
)

as.ngraph(x, ...)

# S3 method for data.frame
as.ngraph(x, directed = TRUE, ...)

# S3 method for neuron
as.ngraph(x, directed = TRUE, method = c("swc", "seglist"), ...)

## Arguments

el

A two column matrix (start, end) defining edges. start means closer to the root (soma) of the neuron.

vertexnames

Integer names for graph nodes - the edge list is specified using these names (see details).

xyz

3D coordinates of vertices (optional, Nx3 matrix, or Nx4 matrix when 4th column is assumed to be diameter)

diam

Diameter of neuron at each vertex (optional)

directed

Whether the resultant graph should be directed (default TRUE)

weights

Logical value indicating whether edge weights defined by the 3D distance between points should be added to graph (default FALSE) or a numeric vector of weights.

vertex.attributes, graph.attributes

List of named attributes to be added to the graph. The elements of vertex.attributes must be vectors whose length is compatible with the number of elements in the graph. See set.vertex.attribute for details.

x

Object to convert (see method descriptions)

...

Arguments passed to methods

method

Whether to use the swc data (x$d) or the seglist to define neuronal connectivity to generate graph. ## Value an igraph object with additional class ngraph, having a vertex for each entry in vertexnames, each vertex having a label attribute. All vertices are included whether connected or not. ## Details Note that the as.ngraph.neuron method always keeps the original vertex names (a.k.a. PointNo) as read in from the original file. ## Connectivity We make the following assumptions about neurons coming in • They have an integer vertex name that need not start from 1 and that may have gaps. This is analogous to the PointNo field of the core data block of neuron objects. • The edge list that defines connectivity specifies those edges using pairs of vertex names, _not_ raw vertex indices. We make no attempt to determine the root points at this stage. The raw vertex ids in the graph will be in the order of vertexnames and can therefore be used to index a block of vertex coordinates. The vertexnames will be stored using the vertex attribute name. The underlying igraph class allows nodes to be specified by their name. This provides a convenient way to define nodes in an ngraph object by the numeric identifier encoded by the PointNo field of the corresponding neuron. When the graph is directed (default) the edges will be from the root to the other tips of the neuron. ## Morphology The morphology of the neuron is encoded by the combination of connectivity information (i.e. the graph) and spatial data encoded as the 3D position and diameter of each vertex. Position information is stored as vertex attributes X, Y, and Z. ## See also igraph, set.vertex.attribute, subset.neuron for example of graph-based manipulation of a neuron, plot3d.ngraph Other neuron: neuron(), plot.dotprops(), potential_synapses(), prune(), resample(), rootpoints(), spine(), subset.neuron() ## Examples n=Cell07PNs[[1]] g=as.ngraph(n) library(igraph) #> #> Attaching package: ‘igraph’ #> The following object is masked from ‘package:nat’: #> #> union #> The following object is masked from ‘package:testthat’: #> #> compare #> The following objects are masked from ‘package:stats’: #> #> decompose, spectrum #> The following object is masked from ‘package:base’: #> #> union # check that vertex attributes of graph match X position all.equal(V(g)$X, n$d$X)
#> [1] TRUE

# Use 3D segment lengths as edge length of graph
gw=as.ngraph(n, weights=TRUE)
# find longest path across graph
d=get.diameter(gw)
# make a new neuron using the longest path
gw_spine=as.neuron(induced.subgraph(gw, d))
# make a new neuron containing all nodes except those in longest path
gw_antispine=as.neuron(delete.vertices(gw, d))

# note use of bounding box of original neuron to set plot axes
plot(gw_spine, col='red', boundingbox=boundingbox(n))