Find the input/output partners for a flywire neuron

flywire_partners is a low level function returning one row for every synapse.

flywire_partner_summary summarises the connectivity of one or more flywire neurons.

Usage

flywire_partners(
  rootids,
  partners = c("outputs", "inputs", "both"),
  details = FALSE,
  roots = TRUE,
  reference = c("either", "FAFB14", "FlyWire"),
  cloudvolume.url = NULL,
  method = c("auto", "spine", "sqlite"),
  Verbose = TRUE,
  local = NULL,
  ...
)

flywire_partner_summary(
  rootids,
  partners = c("outputs", "inputs"),
  threshold = 0,
  remove_autapses = TRUE,
  cleft.threshold = 0,
  summarise = FALSE,
  surf = NULL,
  version = NULL,
  timestamp = NULL,
  method = c("auto", "spine", "sqlite", "cave"),
  Verbose = NA,
  local = NULL,
  ...
)

Arguments

rootids: Character vector specifying one or more flywire rootids. As a convenience for flywire_partner_summary this argument is passed to flywire_ids allowing you to pass in data.frames, flywire URLs or cell type queries.
partners: Whether to fetch input or output synapses or both.
details: Whether to include additional details such as X Y Z location (default FALSE)
roots: Whether to fetch the flywire rootids of the partner neurons (default TRUE)
reference: A character vector or a templatebrain object specifying the reference template brain for any 3D coordinate information. The default value of "either" will use the natural reference space of the data source (FAFB14 for SQLite tables, FlyWire for the spine service).
cloudvolume.url: The segmentation source URL for cloudvolume. Normally you can ignore this and rely on the default segmentation chosen by choose_segmentation
method: Whether to use a local SQLite database or remote spine service for synapse data. The default auto uses a local database when available (45GB but faster).
Verbose: Whether to print status messages
local: path to SQLite synapse data. Evaluated by fafbseg:::local_or_google. Work in progress. Default is to download this data and place it in ~/projects/JanFunke.
...: Additional arguments passed to pbsapply
threshold: For flywire_partner_summary only return partners with greater than this number of connections to the query neuron(s) (default of 0 returns all connections)
remove_autapses: For flywire_partner_summary whether to remove autapses (defaults to TRUE)
cleft.threshold: A threshold for the cleft score calculated by Buhmann et al 2019 (default 0, we have used 30-100 to increase specificity)
summarise: Whether to collapse down the results for multiple query neurons into a single entry for each partner neuron.
surf: An object defining a 3D ROI inside which the presynaptic position must be located. Can be a mesh3d object, or any object which as.mesh3d can handle including hxsurf and boundingbox objects. See pointsinside for details.
version: Integer materialisation version
timestamp: A timestamp to normalise into an R or Python timestamp in UTC. The special value of 'now' means the current time in UTC.

Value

A data.frame with a regtemplate attribute specifying the reference brain space for any xyz points. Columns vary slightly depending on whether data is fetched from spine/ITANNA or a local sqlite database. The more obscure ones include:

prepost When partners = "both" then this column will be present. For any given row (synapse), prepost=1 when the initial query neuron is downstream (postsynaptic) and the partner is upstream.
score came straight from Buhmann et al and is supposed to indicate some confidence score for the predicted synapse.
cleft_score is the more useful one and was a later addition by Stephan Gerhard that asks whether the pre and post synapses are positioned at sensible distances on each side of a cleft defined by a separate neural network from Larissa Heinrich in Stephan Saalfeld’s group.

Details

FIXME behaviour when there are no partners is not well-defined.

Note that there are many duplicated connections in this raw output, false autapses (i.e. the same neuron connected to itself) and other false positives. See Buhmann et al for details and ideas about cleaning up the results.

Also note that the ids returned are of the class integer64 for flywire_partners where there is one row for each synapses/connection; but for flywire_partner_summary where rows report the number of connections between pairs of neurons, they are of type character. This is because the integer64 type is more compact but less robust because it is not a base R type but instead provided by the bit64 package. Some R functions such as sapply strip the class from integer64 vectors, treating them as doubles of a completely different value.

flywire_partners and flywire_partner_summary by default report on the active connectivity state of neurons. At present only flywire_partner_summary allows time travel to historic materialisations using the version or timestamp arguments (see flywire_timestamp for details). This support actually depends on the cave backend (which will automatically be selected when method='auto').

Examples

# \donttest{
pp=flywire_partners("720575940621039145")
#> Warning: /home/runner/projects/JanFunke//flywire_synapses.db does not exist
#> Fetching supervoxel ids for id: 720575940621039145
#> Finding synapses for supervoxels
#> Reading synapse data
#> Fetching root ids
head(pp)
#>    offset    scores cleft_scores          pre_svid         post_svid
#> 1  152196  9.542592          117 81491472725509269 81491472725509199
#> 2  828599 93.800766            0 81561978908660664 81561978908653994
#> 3  828625 93.588776            1 81561978908669037 81561978908667521
#> 4 1236913 54.307476            1 81631042385463742 81631042385462154
#> 5 1236914 33.748131            0 81631042385459399 81631042385454876
#> 6 1236915  6.670522            0 81631042385462087 81631042385470852
#>              post_id             pre_id
#> 1 720575940623607372 720575940621039145
#> 2 720575940632985261 720575940621039145
#> 3 720575940589754844 720575940621039145
#> 4 720575940623607372 720575940621039145
#> 5 720575940623607372 720575940621039145
#> 6 720575940623607372 720575940621039145
class(pp$post_id)
#> [1] "integer64"
# }
# \donttest{
# Note that post_id is of type character
flywire_partner_summary("720575940621039145", partners='out')
#> Warning: /home/runner/projects/JanFunke//flywire_synapses.db does not exist
#> Fetching supervoxel ids for id: 720575940621039145
#> Finding synapses for supervoxels
#> Reading synapse data
#> Fetching root ids
#> # A tibble: 1,214 × 3
#>    post_id            weight     n
#>    <chr>               <int> <int>
#>  1 720575940623607372    891     1
#>  2 720575940639232858    172     1
#>  3 720575940622838154     62     1
#>  4 720575940631200327     62     1
#>  5 720575940631914700     34     1
#>  6 720575940628412732     31     1
#>  7 720575940619553671     30     1
#>  8 720575940621000831     29     1
#>  9 720575940611428761     27     1
#> 10 720575940624743946     27     1
#> # ℹ 1,204 more rows
flywire_partner_summary("720575940621039145", partners='in')
#> Warning: /home/runner/projects/JanFunke//flywire_synapses.db does not exist
#> Fetching supervoxel ids for id: 720575940621039145
#> Finding synapses for supervoxels
#> Reading synapse data
#> Fetching root ids
#> # A tibble: 619 × 3
#>    pre_id             weight     n
#>    <chr>               <int> <int>
#>  1 720575940623607372    891     1
#>  2 720575940613380914     90     1
#>  3 720575940631842360     88     1
#>  4 720575940621000831     77     1
#>  5 720575940613056149     74     1
#>  6 720575940631200327     69     1
#>  7 720575940624377224     62     1
#>  8 720575940613603737     60     1
#>  9 720575940635047672     57     1
#> 10 720575940621717469     56     1
#> # ℹ 609 more rows
flywire_partner_summary("720575940621039145")
#> Warning: /home/runner/projects/JanFunke//flywire_synapses.db does not exist
#> Fetching supervoxel ids for id: 720575940621039145
#> Finding synapses for supervoxels
#> Reading synapse data
#> Fetching root ids
#> # A tibble: 1,214 × 3
#>    post_id            weight     n
#>    <chr>               <int> <int>
#>  1 720575940623607372    891     1
#>  2 720575940639232858    172     1
#>  3 720575940622838154     62     1
#>  4 720575940631200327     62     1
#>  5 720575940631914700     34     1
#>  6 720575940628412732     31     1
#>  7 720575940619553671     30     1
#>  8 720575940621000831     29     1
#>  9 720575940611428761     27     1
#> 10 720575940624743946     27     1
#> # ℹ 1,204 more rows

# summary for neuron at a XYZ location (in this case in raw coordinates)
flywire_partner_summary(flywire_xyz2id(cbind(155682, 58180, 3215),
  rawcoords = TRUE))
#> Warning: /home/runner/projects/JanFunke//flywire_synapses.db does not exist
#> Fetching supervoxel ids for id: 720575940623607372
#> Finding synapses for supervoxels
#> Reading synapse data
#> Fetching root ids
#> # A tibble: 1,213 × 3
#>    post_id            weight     n
#>    <chr>               <int> <int>
#>  1 720575940639232858    172     1
#>  2 720575940622838154     62     1
#>  3 720575940631200327     62     1
#>  4 720575940631914700     34     1
#>  5 720575940628412732     31     1
#>  6 720575940619553671     30     1
#>  7 720575940621000831     29     1
#>  8 720575940611428761     27     1
#>  9 720575940624743946     27     1
#> 10 720575940650971257     27     1
#> # ℹ 1,203 more rows

if (FALSE) {
# Use Ctrl+Shift+J to share a flywire scene and then do this to get partner
# summary for that URL
flywire_partner_summary(clipr::read_clip())

cct=flywire_cave_query('cambridge_celltypes', live = T)
dl1.lh=flywire_partner_summary(cct$pt_root_id[grep("DL1", cct$cell_type)],
  surf=subset(elmr::FAFB14NP.surf, "LH_R"))
  # use a rectangular bounding box around LH instead
dl1.lhbb=flywire_partner_summary(cct$pt_root_id[grep("DL1", cct$cell_type)],
  surf=boundingbox(subset(elmr::FAFB14NP.surf, "LH_R")))
}
# }